Coexpression of the maize delta-zein and beta-zein genes results in stable accumulation of delta-zein in endoplasmic reticulum-derived protein bodies formed by beta-zein.
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چکیده
منابع مشابه
The Maize y-Zein Sequesters a-Zein and Stabilizes Its Accumulation in Protein Bodíes of Transgenic Tobacco Endosperm
Zeins are seed storage proteins that form accretions called protein bodies in the rough endoplasmic reticulum of maize endosperm cells. Four types of zeins, a, p, y , and 6, aggregate in a distinctive spatial pattern within the protein body. We created transgenic tobacco plants expressing a-zein, y-zein, or both to examine the interactions between these proteins leading to the formation of prot...
متن کاملThe maize floury1 gene encodes a novel endoplasmic reticulum protein involved in zein protein body formation.
The maize (Zea mays) floury1 (fl1) mutant was first reported almost 100 years ago, but its molecular identity has remained unknown. We report the cloning of Fl1, which encodes a novel zein protein body membrane protein with three predicted transmembrane domains and a C-terminal plant-specific domain of unknown function (DUF593). In wild-type endosperm, the FL1 protein accumulates at a high leve...
متن کاملZein protein interactions, rather than the asymmetric distribution of zein mRNAs on endoplasmic reticulum membranes, influence protein body formation in maize endosperm.
Prolamin-containing protein bodies in maize endosperm are composed of four different polypeptides, the alpha-, beta-, gamma-, and delta-zeins. The spatial organization of zeins within the protein body, as well as interactions between them, suggests that the localized synthesis of gamma-zeins could initiate and target protein body formation at specific regions of the rough endoplasmic reticulum....
متن کاملEthanolic extraction of zein from maize
Zein can be extracted from maize (Zea mays) using ethanol solutions, and recovered from the extract by diluting with water. Dilution to 40% ethanol will precipitate a mixture of zein and maize lipid with 15% lipid. For possible zein applications, such as film production, purer zein is required. Most of the lipid can be removed from the precipitate by selective re-extraction with hexane or ethan...
متن کاملHeterogeneity of zein mRNA and protein in maize.
Zein, the prolamine fraction of maize, is localized in the endosperm in membrane-bound structures called protein bodies, which have polyribosomes on their surfaces. These polysomes or the mRNA fraction isolated from them will direct the synthesis of zein-like proteins in vitro. The in vitro products consist primarily of two molecular weight classes but show considerable charge heterogeneity whe...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1997
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.9.9.1683